Explore More Effective Viral CPE Assays for Virology and Vaccine Research
Download the Vaccine & Virology Handbook to discover a more efficient method to quantitatively characterize viral samples
Developing novel methods to screen antivirals is important for creating broad-spectrum anti-viral drugs and vaccines. The accurate measurement of viral activity plays an essential role for viral vaccine development and infectious disease studies. Find out why the rapid, sensitive, and high throughput xCELLigence Real-Time Cell Analysis Assay is ideal for infectious viral assays.
Applications in the Vaccine & Virology Handbook include:
- Viral Titer Determination
- Detection and Quantification of Neutralizing Antibodies
- Studying Anti-viral Drugs
- Testing Virucides
- Oncolytic Viruses
- Assessing virus quality/fitness
Say Goodbye to Endpoint Assays:
Conventional methods to measure virus and neutralizing antibody titers such as plaque assays, plaque reduction neutralization tests (PRNT), immunofluorescence foci assays (IFA), or interferon CPE assays are:
- Labor intensive
- Time consuming
- Difficult to reproduce
- No assessment or quantification of the full virus life cycle.
- Wrong timepoint can result in inaccurate calculation of viral titer and lytic activity
With the xCELLigence Real-Time Cell Analysis Assay:
- Monitor viral cytopathic effects in unprecedented detail without the use of agar, dye or fixative over time scales ranging from minutes to days
- Quantitatively assess the efficacy of a particular vaccine
- Greatly reduce work load and manual handling of samples, ideal for infectious material.
- Obtain quantitative kinetics for virus infection with exquisite sensitivity and reproducibility
Sample Data in this Handbook:
Figure 7. Using Agilent xCELLigence RTCA to determine viral titer. (A) Upper panel: real-time
monitoring of WNV-induced cytopathic effect in Vero cells. The normalized Cell Index is shown
for E-Plate wells that were inoculated with a negative control (Ctrl) or different numbers of
plaque forming units (PFUs) of WNV. Each curve is an average of two independent replicate
wells. The horizontal line denotes the point at which CI has dropped to 50% of its initial value
(before virus addition). The time required to reach this point is referred to as CIT50. Lower panel:
by plotting CIT50 as a function of viral titer, a standard curve was produced, which can be used
for determining virus concentration in diverse types of samples. (B) Real-time monitoring of
SLEV‑induced cytopathic effect in Vero cells. Experimental details and data processing are similar
to part (A).
Figure reprinted from Journal of Virological Methods, volume 173(2), Fang, Y. et al., "Real-Time
Monitoring of Flavivirus Induced Cytopathogenesis Using Cell Electric Impedance Technology,"
pages 251–8. Copyright 2011, with permission from Elsevier.